Which statement is FALSE?1. Flexible domain of T7 RNAP is part of a single protein and it binds to a major grove recognizing T7
phage promoter2. T7 RNAP activity is stringently regulated3. T7 RNAP synthesizes RNA at a rate of 200 nt/sec4. T7 RNAP is a minimal enzyme, which recognizes only one promoter
The high movement of T7 RNA polymerase has made the T7 RNA polymerase-based articulation framework exceptionally amazing for significant level articulation of recombinant protein. In any case, the overactivity of T7 RNA polymerase would likewise realize negative consequences for plasmid security and protein creation, particularly while communicating a lethal protein. In the event that the last job is predominant, it is important to embrace a few measures to weaken the action or the measure of T7 RNA polymerase in the cells
This represents an issue when an engineered hereditary framework is presented. At the point when it depends on the interpretation and interpretation hardware of the host, it turns out to be verifiably implanted in their guideline, causing it delicate to changes that to happen during cell development and capacity
Hence, the right answer is option 2 "T7 RNAP activity is strengthly regulated"
<u>A cell wall containing peptidoglycan and distinctive ribosomes</u>
Bacterial cell dividers are made of <em>peptidoglycan</em> (additionally called murein), which is produced using<em> polysaccharide chains cross-connected by irregular peptides containing D-amino acids</em>
Gram-positive microscopic organisms have a thick cell divider containing numerous layers of <em>peptidoglycan and teichoic acids</em>
<em>Ribosomes are small particles</em> which is related proteins that capacity to synthesize proteins and comprising of RNA
Proteins are needed for many cellular capacities, for example, directing chemical procedures and repairing damage
Ribosomes can be discovered drifting inside the appended to the endoplasmic reticulum or cytoplasm
Afferent nerve fibers carry signals from the somatic receptors to the posterior horn of the spinal cord or to the brainstem. So i beleive it is 4 but not too sure. Hope this helped a bit (:
