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QveST [7]
3 years ago
13

Why does coomassie brilliant blue r interact differently with different proteins?

Biology
1 answer:
alexandr1967 [171]3 years ago
5 0
Dimethyl sulfoxide was found to be effective for extraction of Coomassie Brilliant Blue R-250 (Coomassie R) from stained proteins on polyacrylamide gel slices. A good correlation was found between the ability of different proteins to bind Coomassie R and their capacity for interaction with Coomassie Brilliant Blue G-250 (Coomassie G) in solution. Scatchard analysis showed that the number of Coomassie R ligands bound to each protein molecule is approximately proportional to the number of positive charges on the protein, about 1.5-3 dye. I hoped I help you and good Luke and I know it’s night am sorry I just love helping people and I ate to much sugar
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