Dimethyl sulfoxide was found to be effective for extraction of Coomassie Brilliant Blue R-250 (Coomassie R) from stained proteins on polyacrylamide gel slices. A good correlation was found between the ability of different proteins to bind Coomassie R and their capacity for interaction with Coomassie Brilliant Blue G-250 (Coomassie G) in solution. Scatchard analysis showed that the number of Coomassie R ligands bound to each protein molecule is approximately proportional to the number of positive charges on the protein, about 1.5-3 dye. I hoped I help you and good Luke and I know it’s night am sorry I just love helping people and I ate to much sugar
Since only starch have alpha glycosidic bond among all other macromolecules, and only amylase is appear to dissolve the storage material in the seed so the storage material is starch as a source of carbon and energy.
