Answer:
A breakdown of the breaking buffer was first listed with its respective component and their corresponding value; then a table was made for the stock concentrations in which the volume that is being added was determined by using the formula 
. It was the addition of these volumes altogether that make up the 0.25 L (i.e 250 mL) with water
Explanation:
Given data includes:
Tris= 10mM
pH = 8.0
NaCl = 150 mM
Imidazole = 300 mM
In order to make 0.25 L solution buffer ; i.e (250 mL); we have the following component.
Stock Concentration Volume to be Final Concentration
added
1 M Tris 2.5 mL 10 mM
5 M NaCl 7.5 mL 150 mM
1 M Imidazole 75 mL 300 mM
. is the formula that is used to determine the corresponding volume that is added for each stock concentration
The stock concentration of Tris ( 1 M ) is as follows:
.
The stock concentration of NaCl (5 M ) is as follows:
.
The stock concentration of Imidazole (1 M ) is as follows:
.
Hence, it is the addition of all the volumes altogether that make up 0.25L (i.e 250 mL) with water.
Answer:
1.40*10⁻² M
Explanation:
We have the solubility formula
Solubility,
S = KH*P
where
KH = measure of hardness of water / carbonate hardness = 3.50*10⁻² mol/L.atm
P = atmospheric pressure = 0.400 atm
Hence, we have
S = KH*P
= (3.50*10⁻² mol/L.atm)*(0.400 atm)
= 1.40*10⁻² mol/L
But 1 mol/L = 1 M,
Hence, the answer (1.40*10⁻² mol/L
) is equivalent to
= 1.40*10⁻² M
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