It was the famous Theodor Escherich who is a famous German bacteriologist that discovered the E. coli and was also known for his research in the area of protein synthesis. In addition to that, Theodor Escherich discovered the bacteria type when he examined its samples from a patient suffering from gastroenteritis.
The stem of the plant should be cut at least three to four centimeters above the surface level.
The stem should be tightly fixed into the glass capillary.
All connection should be made air-tight by using paraffin.
Initial and final readings should be carefully noted.