Answer:
The correct option is A, since if the DNA enters in a circular form it is called a plasmid, and the plasmids upon entering the bacteria self-replicate outside the genetic structure, which gives the bacteria better survival qualities.
Explanation:
Plasmids are generally circular extrachromosomal DNA molecules that replicate and transmit independently of chromosomal DNA, and are normally present in bacteria, archaea, and sometimes in eukaryotic organisms such as yeast. Its size varies from 3 to 10 kb. The number of plasmids can vary, depending on their type, from a single copy to a few hundred per cell. Plasmid vectors allow cloning of exogenous DNA ligands of up to 4 kb since a larger size than this makes cloning in these vectors difficult. The term plasmid was first introduced by the American molecular biologist Joshua Lederberg in 1952. Plasmids can only coexist as one or more copies in each bacterium, due to cell division they can be lost in one of the secreted bacteria.
Yellow stripes because it is dominant.
Answer:
The correct answer is b.Amplify the gene using PCR. Insert the gene into a plasmid vector. Transform the vector into the bacteria.
Explanation:
If I have a very small amount of gene for a fluorescent protein than the first step is to amplify the gene so that appropriate protein can be produced. PCR is the instrument that is used to amplify the protein.
So after amplification of the gene, the plasmid vector will be used in which the gene will be inserted because the plasmid vector is used to insert this gene in host cells where protein will be expressed.
The final step will be to transform bacteria with recombinant plasmid so that plasmid can make its copy and express a fluorescent protein in bulk.
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