<span>A series of complexes from electron
donors to acceptors happen in an electron transport chain. Due to the pumping action in the chain,
protons result to having a high concentration in the intermembrane space yet a
low concentration in the mitochondrial matrix.</span>
A pyramid of energy can never be inverted because energy decreases by tenfold each level. This can't be reversed because energy can't just increase by tenfold. It has to come from somewhere.