Peptides can be separated using an ion-exchange column based on their isoelectric (pl) values. At which pH values would two diff
erent peptides, one with a pl of 5.0 and the other with a pi of 9.4, bind to a caution- and anion-exchange column? Each peptide may be capable of binding to each column at more than one pH value. anion-exchange column at pH = 3.0__________pH = 7.1__________ pH = 10.1_____________ cation-exchange column at pH = 3.0____________ pH = 7.1___________pH = 10.1___________
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