A Negative stain such as India ink or Congo red-Look for the presence of a capsule.
This stain method is usually used to stain the area around the microorganism.
B The Gram stain-Divide bacteria into two groups based on cell wall structure (thick vs. thin).
This is type of differential staining used to distinguish organisms based on their staining properties. Gram + and Gram- bacteria stain different because of different cell wall structure.
C The Ziehl-Neelsen Acid-fast stain- Identify bacteria with waxy cell walls such as: Mycobacterium tuberculosis (the cause of TB).
This is also differential staining method which uses heat and phenol to derive dye into the cells with lipid-rich walls.
D Simple stain with a basic dye-Stain microbes a bright color to make it easier to see them in bright field microscopy.
One dye is used in simple staining in order to determinate the size, shape and arrangement of the cells.
E The Schaeffer-Fulton Endospore stain-Identify Bacillus or Clostridium species, such as the causative agents of anthrax, botulism, tetanus and gangrene.
This is a special type of staining only used for the bacteria that can form endospores. Bacteria are first treated with heat and then with malachite green, which is very strong stain that can penetrate endospores.
Answer an unit of measurement.
For example: inch, hour, minutes, feet, meters.
Out of the following choices given, the true statement about diffusion is molecules move from areas of high concentration to areas of low concentration. The correct answer will be D.
Nucleotide bases are added to DNA strands in a 5' to 3' direction.
What is DNA replication?
When a cell divides, DNA copies itself through a process called replication.
- Generally speaking, DNA replication involves uncoiling the helix, strand separation by rupturing the hydrogen bonds between the complementary strands, and synthesis of two new strands through complimentary base pairing.
- The origin of replication, which is a precise location in the DNA, is where replication starts.
- From the point of origin, DNA replication is bidirectional.
- The two parent DNA strands unwind and split apart at the origin of replication to generate two "Y-shaped" replication forks in order to start DNA replication.
- These unwinding enzymes are known as DNA helicases.
- The real location of DNA copying is at these replication forks.
- Proteins that destabilize helices bind to the single-stranded areas to prevent the two strands from rejoining.
- To reduce stress on the helical molecule during replication, enzymes known as topoisomerases cause breaks in the DNA and then reunite them.
- The hydrogen bonding of free DNA nucleotides with those on each parent strand results in the formation of new complementary strands as the strands continue to unwind and split in both directions around the entire DNA molecule.
- The new nucleotides are joined by DNA polymerases using phosphodiester bonds as they align themselves opposite each parent strand using hydrogen bonds.
- Deoxynucleotide triphosphates, which are made up of a nitrogenous base called deoxyribose and three phosphates, are the actual nucleotides that are aligning through complementary base pairing.
- Two of the phosphates are withdrawn to provide energy for bonding as the phosphodiester bond forms between the 3' OH of the previous nucleotide in the DNA strand and the 5' phosphate group of the next nucleotide.
- In the end, two identical DNA molecules are created when each parent strand acts as a template for the synthesis of a complementary copy of itself.
Hence, nucleotide bases are added to DNA strands in a 5' to 3' direction.
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