Question

A researcher measured the absorbance and % transmittance of a blue dye at light wavelengths between 425 and 700 nm. the measurements were made every 25 nm in a spectrophotomter. how should she plot her data so she can determine the wavelengths of maximum absorbance and % transmittance?

Answer 1

Answer;

Plot the absorbance and the % transmittance on the y-axis, and the wavelength in nm on the x-axis.

Explanation;

Many compounds absorb ultraviolet (UV) or visible (Vis.) light. The amount of radiation absorbed may be measured in a number of ways:

• Transmittance, T = P / P0
• % Transmittance, %T = 100 T
• Absorbance,  A = log10 P0 / P;
• etc.

In this case; the wavelengths of maximum absorbance and % transmittance can be determined by Plotting the absorbance and the % transmittance on the y-axis, and the wavelength in nm on the x-axis.

Answer 2

She should plot her data where the y axis contains the absorbance values while the x-axis are the wavelengths. It should be that the dependent variable is always at the y-axis and the independent variable or the measurement that is manipulated is at the y-axis. Hope this helps.