Question

Which procedure would help ensure that a new piece of DNA can be inserted inside a bacterial plasmid when the two are mixed together?
The DNA fragment is pretreated with DNA ligase.
The fragment and plasmid are both cut with the same restriction enzyme.
The plasmid is pretreated with DNA ligase.
The plasmid is modified to contain GFP.

Answer 1

The fragment and plasmid are both cut with the same restriction enzyme is the that help ensure that a new piece of DNA can be inserted inside a bacterial plasmid when the two are mixed together.

Explanation:

The gene cloning requires one of the necessary step of forming chimera in which gene or DNA sequence of interest in inserted into the plasmid of bacteria.

The gene of interest and plasmid is cut by using same endonuclease enzymes.

The endonuclease used are same because they produce fragments having same complementary ends which bond to form chimera or recombinant DNA.

The overhangs produced by endonuclease lets the gene and plasmid form base pair with each other.

If overhangs are not there DNA ligase performs the function of sealing the plasmid and gene.