Answer:
Background
During the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. We have developed a method based on a modified broad-range PCR and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. The broad-range PCR primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrB and parE. The primer design allowed the use of a novel DNA amplification method, which produced labeled, single-stranded DNA suitable for microarray hybridization. The probes on the microarray were designed from the alignments of species- or genus-specific variable regions of the gyrB and parE genes flanked by the primers. We included mecA-specific primers and probes in the same assay to indicate the presence of methicillin resistance in the bacterial species. The feasibility of this assay in routine diagnostic testing was evaluated using 146 blood culture positive and 40 blood culture negative samples.
Explanation:
Results
Comparison of our results with those of a conventional culture-based method revealed a sensitivity of 96% (initial sensitivity of 82%) and specificity of 98%. Furthermore, only one cross-reaction was observed upon investigating 102 culture isolates from 70 untargeted bacteria. The total assay time was only three hours, including the time required for the DNA extraction, PCR and microarray steps in sequence.
Portals of entry are the sites where micro-organisms can enter a host and reproduce, causing diseases or infections. There are various portals of entry in the human body. This can include the skin, our mucous membranes, and even our respiratory and gastrointestinal tracts. Portals of exit are where these micro-organisms leave and spread to other individuals. Portals of exit include coughing, sneezing, and some of our bodily fluids.
Answer:
Corrigiendo el error de Schwann y preparando el principio de Virchow, señaló que las células se multiplican por escisión de su núcleo, no por generatio aequivoca a partir del protoplasma originario. ... Según los historiadores Pagel (1945) y Ackerknecht (1957), parece que Remak precedió a Virchow en sus descubrimientos.
Explanation:
¡espero que esto ayude!
Answer:
The Power of Food
Cholesterol can combine with fat, calcium, and other substances in the blood to form plaque. Plaque then slowly builds up and hardens in the arteries, causing them to narrow. This buildup of plaque, a condition called atherosclerosis, can lead to heart disease, heart attack, and stroke.
Explanation:
pa brain lest thank you°^°
<span> Romanticism author usually are famous for the picture they sketch with their words about nature and beauty
so He would like to write about the
</span><span>the beauty of a weeping willow
</span>so i conclude option C is correct
hope it helps
