Researchers studying yeast discovered that, for some mutants, when the temperature at which the cells are grown is elevated from
25ºC to 37ºC, their secretory pathway no longer functions and the cells grow dense with unsecreted protein. When these cells are examined microscopically, they can be divided into groups that vary in terms of where the unsecreted proteins accumulate. In some of the mutants, proteins accumulate in the ER; in others, the Golgi; in others, they accumulate in vesicles near the plasma membrane. What is the likely explanation for this difference in appearance? Choose one:
The temperature-sensitive mutant proteins accumulate in different compartments
Different temperature-sensitive mutations promote an increase in protein synthesis.
Different temperature-sensitive mutations disrupt protein synthesis.
Different temperature-sensitive mutations disrupt the integrity of cell membranes.
Different temperature-sensitive mutations affect different stages of the transport process.
In the mentioned case, the gathering of unsecreted protein has been witnessed in distinct locations, that is, in the endoplasmic reticulum, in the vesicles near the plasma membrane, and in the Golgi complex. Thus, it can be stated that the unsecreted protein will get gathered at a particular location at a particular temperature.
Hence, the correct statement is that different temperature-sensitive mutations influence different phases of the transport process.
