I have done some research as well as asking some friends. This is what I came up with. Not sure if it is 100% correct.
step = time after death
1. Livor Mortis = 20-30 minutes
2. Eyes film (cloud) = 2 hours
3. Rigor Mortis begins = 4-6 hours
4. Empty small intestines = 12 hours
5. Bloating = 3-5 days
6. Decay
Explanation:
According to the Henderson-Hasselbalch equation, the relation between pH and
is as follows.
pH = 
where, pH = 7.4 and
= 7.21
As here, we can use the
nearest to the desired pH.
So, 7.4 = 7.21 + 
0.19 = 
= 1.55
1 mM phosphate buffer means
+
= 1 mM
Therefore, the two equations will be as follows.
= 1.55 ............. (1)
+
= 1 mM ........... (2)
Now, putting the value of
from equation (1) into equation (2) as follows.
1.55
= 1 mM
2.55
= 1 mM
= 0.392 mM
Putting the value of
in equation (1) we get the following.
0.392 mM +
= 1 mM
= (1 - 0.392) mM
= 0.608 mM
Thus, we can conclude that concentration of the acid must be 0.608 mM.
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