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mr Goodwill [35]
3 years ago
10

Carbonic anhydrase of erythrocytes (Mr 30,000) has one of the highest turnover numbers known. It catalyzes the reversible hydrat

ion of H2O + CO2 ⇄H2CO3 This is an important process in the transport of CO2 from the tissues to the lungs. If 10.0 g of pure carbonic anhydrase catalyzes the hydration of 0.30 g of CO2 in 1 min at 37 C at Vmax, what is the turnover number (kcat ) of carbonic anhydrase (in units of min-1 )?
Chemistry
1 answer:
Afina-wow [57]3 years ago
6 0

Explanation:

According to the given data, the turnover number can be calculated as follows.

      Turnover number = K_{cat} = \frac{V_{max}}{\text{Concentration of enzyme}}

     V_{max} = \frac{\text{Moles of CO_{2} hydrolyzed}{second}

Therefore, moles of CO_{2} hydrolyzed is as follows.

Moles of CO_{2} hydrolyzed = \frac{Mass of CO_{2}}{Molar mass of CO_{2}}

                 = \frac{0.30}{44}

                 = 0.00682 moles

Now, moles of CO_{2} hydolyzed per second is calculated as follows.

Moles of CO_{2} hydolyzed per second = \frac{0.00682}{60}

             = 1.137 \times 10^{-4} moles/second = V_{max}

And,

Moles of enzyme = \frac{Mass}{\text{Molar mass}}

                       = \frac{10.0 \mu g}{30000}

                       = 3.33 \times 10^{-10} moles

Therefore, the value of K_{cat} is as follows.

    K_{cat} = \frac{1.137 \times 10^{-4} moles}{3.333 \times 10^{-10} moles}

               = 0.3411 \times 10^{6} per second

               = 0.3411 \times 60 \times 10^{6} per minute

               = 20.466 \times 10^{6} per minute

Thus, we can conclude that the turnover number (K_{cat}) of carbonic anhydrase (in units of min^{-1}) is 20.466 \times 10^{6} per minute.

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