Molarity = (Mass/ molar mass) x (1/ volume of solution in Litres)
Mass = Molarity x molar mass x volume of solution in Litres
Molarity of Tris = 100 mM = 0.1 M
volume of Tris sol. = 100 mL = 0.1 L
molar mass of Tris = 121.1 g/mol
Hence,
mass of Tris = Molarity of Tris x molar mass ofTris x volume of Tris solution
= 0.1 M x 121.1 g/mol x 0.1 L
= 1.211 g
mass of Tris = 1.211 g
There are a lot of separation processes. To name a few, these can be distillation, centrifugation, extraction, membrane or sorption process and many other. To know which is the best technique, you should know the property between two substances that have a stark difference. In this case, it is the polarity. Ethyl alcohol is more polar than ethyl ester and less dense. Thus, these two won't mix. So, take advantage of their density difference by decantation or centrifugation.
Lipid you can look up the chemical structures and lipids are the longest which make them the largest
