Answer:
DNA ligase
Explanation:
DNA replication is an enzyme mediated, 3-step process during which the DNA molecule produces a copy of itself. The 3 steps involved are as follows;
- initiation
- elongation
- termination
<em>Initiation </em>involves the unwinding of the double helix structure of the DNA using DNA helicase enzyme
<em>Elongation</em> involves binding of RNA primer to the DNA strands and addition of bases to the primer<em> </em>to elongate the new chains. Bases are added to the leading strand continuously while the lagging strand is replicated in short segments (okazaki segments).
<em>Termination</em> involves the unbinding of RNA primer and substitution of its bases by DNA bases. The Okazaki fragments are then joined together using the DNA ligase enzyme.
<em>The short, numerous segments of DNA observed in the mixture is the Okazaki fragments of the lagging strand. This is due to the absence of the enzyme involved in the joining of these fragments.</em>
Hence, the answer is DNA ligase.
The answer to the question is A
Answer:
The correct answer is c. detergent is used to break apart plant cells to extract DNA.
Explanation:
Here we are talking about DNA isolation in E.coli and detergent is used to break apart the bacterial cell membrane by destabilizing the cell membrane. Detergent disintegrates the proteins present in the cell membrane which causes the rupture of membrane releasing the cell extract outside the cell.
Detergent is not able to break plant cell because it is made up of cellulose and require blending or mashing. Detergent can only be used to disintegrate the cell membrane because only cell membranes contain lipids and proteins.
Hence, the correct answer is c. detergent is used to break apart plant cells to extract DNA.
In general, echinoderms typically respire by simple diffusion, using gills or specialized projections, like tube feet or pockets, to circulate water and oxygen through their bodies. Many echinoderms also use a simple hemal system, a series of pockets and tubes that serves almost like a net of veins and arteries.
