<h3>Oxidative Phosphorylation</h3>
Explanation:
The process by which ATP is generated by utilizing the large amount of energy released during the transfer of electrons from NADH or FADH₂ to the O₂ through a series of electron carrier present in the mitochondrial membrane is called oxidative phosphorylation.
The process of electron transfer is accompanied by the pumping of protons across inner membrane of mitochondria, from the mitochondrial matrix to intermembrane space establishing electrochemical proton gradient.
The ATP synthase complex present in the mitochondrial membrane allows the protons to move down the proton gradient as a result enough energy is released to add a phosphate group to ADP and form ATP inside the mitochondrial matrix.
The substrate and the active site both need to fit together perfectly in order for the enzyme to work properly. If their shape is changed, typically through heat or a change in pH, the enzyme will not work.
Creep is a solid material's tendency to deform permanently under pressure or stress.
So, a cracked telephone pole or a broken sidewalk would be examples.
Living organisms use two major types of energy storage. Energy-rich molecules such as glycogen and triglycerides store energy in the form of covalent chemical bonds. Cells synthesize such molecules and store them for later release of the energy.
Answer:
DNA restriction enzymes cut the DNA molecule, while DNA ligases join the resulting DNA fragments
Explanation:
Transformation is a naturally occurring process by which bacteria incorporate exogenous genetic material from their surrounding environment. This process (transformation) is used for DNA cloning via plasmid vectors. In DNA cloning, transformation occurs after restriction enzymes cut the DNA at specific sequences named palindromic sequences (i.e, sequences that can be read the same in opposite direction). Restriction enzymes can generate sticky-ends, where enzymes make staggered cuts in the two strands (e.g., <em>BamH</em>), or blunt ends, where the resulting strands are of the same length (e.g., <em>HaeIII</em>). In general, sticky-end enzymes are more useful because they generate a 3' overhang in one molecule and a complementary 5' overhang in the other, increasing the yield and specificity of ligation. During ligation, a DNA ligase is used to join both DNA strands by forming phosphodiester bonds in the plasmid. Following transformation, bacteria can be selected on antibiotic plates.
