Answer:
1. Designing of the target vector.
2. Insertion of the target vector into the embryonic stem cell
3. Selection of the cells
4. Injecting selected cells into the new cells
5. Breeding of mouse with a normal mouse
Explanation:
The target vectors are made by inserting markers into them. The target vector is inserted into ES cells. The vector generally recombined with the target gene and knock-out that gene. Sometimes, recombination of vectors occurs at incorrect place.
The cells survive in the presence of markers only if the target gene is incorporated with target vector. The cells with the target vector are injected into a normal developing embryo of mouse.
The developed mouse will consist of heterozygous knockout cells and its own cells. The normal mouse is bred with this chimeric mouse. The offspring of these mouse are able to transfer knock-out gene to next generation.
Darwin believed in Darwinism which is a theory of biological evolution stating that all species of organisms arise through a process called natural selection to compete, survive, and reproduce
Answer:
A buffer is a solution that can resist pH change upon the addition of an acidic or basic components. It is able to neutralize small amounts of added acid or base., thus maintaining the pH of the solution relatively stable. This is important for processes and/or reactions which require specific and stable pH ranges.
Explanation:
If you have one sickle cell gene, you have sickle cell trait and you will not develop the sickle cell disease. If you inherit two sickle cell genes, you have sickle cell anemia.
Climate change is already beginning to transform life on Earth. Without action, the impacts of climate change threaten to catastrophically damage our world. But by rallying people around the world to be a part of the solution, together we have the power to limit the effects of climate change.
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