Question

"You must choose between two widely accepted, commonly used colorimetric enzyme immunoassay kits to measure a plasma protein that circulates at a moderately high concentration. Each kit uses a different enzyme and substrate. What is the most important consideration in making your choice?"

Answer 1

Answer:

The correct answer to the question: What is the most important consideration in making your choice? Would be: Wavelenghts detected by microtiter plate reader.

Explanation:

You must bear in mind that the purpose you have here is to measure one plasma protein that circulates at a moderately high concentration in the bloodstream, which means that it will be more challenging to find in samples, than if it were present in high concentrations. Because of this characteristic, you are choosing an immunoassay kit that comes with a colorimetric enzyme, which means that one way in which you will search for the presence of that specific plasma protein, you will need a mechanism that will be able to detect the specific colors and emissions of that specific protein when compared to others that will also show. Microplate readers, also known as photometers, are precisely the machine to use, especially if it is geared towards detection of wavelenghts as it must be remembered that colors have different wavelengths. A machine that can read these wavelenghts will make it easier for you to detect the presence of the plasma protein in comparisson to others in the bloodstream.