Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Answer:
Active transport.
Explanation:
The kidney uses active transport to move these substances from the nephron to the renal vein because these substances did not moves from the nephron bowl to the renal vein through simple diffusion so for this purpose active transport is used in which energy is spent in order to move the substances from one region to another so we can say that kidney must use active transport to move reabsorbed substances from the nephron to the renal vein.
Answer:
Roots---xylem vessel-- upper parts of plant.
Explanation:
The plant absorb water from hairs present on the roots and taken this water to the xylem vessel that is responsible for transporting water to all parts of the plant body. Water enters in the root through a process is called osmosis until it reached to the xylem vessel. Osmosis is a movement of molecules through a semi-permeable membrane into a region of higher solute concentration in order to equalize the solute concentration.
