This is because there might not be one restriction site bordering the gene to be cloned and the identical restriction site in the plasmid. Since these two restriction enzymes make compatible sticky ends, the insert has a chance of combining with the plasmid. To ensure efficient digestion, the two recognition sites should be more than 10 base pairs apart. If one of the enzymes is a poor cutter or if the sites are separated 10 base pairs or less, the digestions <span>should be performed sequentially</span>
Is an organism in its earliest stage of development. In humans, this is the stuff and defined as the developing organism from the fourth day after fertilization to end of the week. After that unborn baby is usually referred to as the fetus.
Answer:
that would be a Jack Crevalle
Answer:
A. student 4
Explanation:
The population of deer increase because with fewer mountain lions, coyotes and bobcats there was less competition within the deer population
