Wait so whats the question?
Answer:
The answer is - C. Use sequencing by ligation protocols
Explanation:
Next Generation Sequencing (NGS) is used to sequence entire genomes such that millions to billions of DNA strands can be sequenced in parallel and therefore reducing the need for fragment cloning methods. Unlike the Sanger sequencing the NGS is a high-throughput and it yields more throughput.
The current use Next Generation Sequencing Platforms use sequencing by ligation protocols which is a DNA sequencing method that uses the mismatch sensitivity of DNA ligase to discover the fundamental sequence of nucleotides in the DNA sequence and it involves the ligation of the probe to a universal primer.
If a bacterium cannot use citrate agar will not change its color (stays green). On the other hand, if bacteria have the ability to use citrate, the medium will change its color from green to blue.
This happens because citrate agar contains pH indicator such as bromothymol blue which transforms from green to blue in alkaline conditions.
It creates extra low visibility and darkness in low spots