I think the correct term to fill in the blank would be "thesis". The main point or central idea that a writer states and then endeavors to prove is called a thesis. It is <span>a statement or theory that is put forward as a premise to be maintained or proved. Hope this answers the question. Have a nice day.</span>
Answer:
1. adopt their husbands' surnames
Explanation:
Married women legally adopt their husband's surname, similar to the Spanish naming customs practised over centuries and until the present.
The surname rules can have some exceptions but, generally speaking, the Hispanics in Latin America will have two given names, including a paternal (primer apellido or apellido paterno) , and the maternal (segundo apellido or apellido materno) surnames.
When women are single, they use both surnames, and once they get married they will take the surname of the husband. This is an accepted and common practice that still exists today.
In some instances, she will be asked to provide her original maternal surname.
Answer:
he was a major general during the french and indian war and supervised the building of fort dobbs
1. Samples of a microbial colony obtained from the natural environment are not pure culture. They are mixed cultures because the environment consists of an array of microorganisms and not one specific organism. A sample obtained from the natural environment such as water or soil may contain several species of microbes that need to be further isolated to obtain a pure culture.
2. Isolation streak plate technique involves the dilution of the sample several folds and then streaking a small amount using a sterile inoculation loop on an agar plate in a zig-zag manner. This is done to obtain a pure culture of bacteria from a mixed population by separating the microprocessor. When the sample is diluted into a small concentration and is streaked onto agar placed, widely spaced, discrete colonies of bacteria can be observed. These isolated colonies can be further cultured to obtain pure cultures by picking and re-streaking them on fresh agar plates.
3. A microbial colony is considered a pure culture because it consists of single species. A single species of bacteria cluster and form an isolated colony on an agar plate which can be further plated on fresh medium.
3-1 - If your agar plate shows discrete uncluttered colonies then you have obtained an isolated colony. The following picture might help you to understand.
3-2. If you subculture isolated microbes into a sterile nutrient broth without any contamination, it will still be considered pure culture because it is single isolated species in a sterile aseptic environment of the nutrient broth.
3.3. A pure culture can become a mixed culture due to contamination. This could be caused by airborne bacteria if your conditions are not aseptic. Contamination is possible if the work area is not clean and sterile or if the glassware is not sterile. The contamination can transform your pure culture into a mixed culture and is hence problematic. So, it is very important to maintain a sterile and aseptic environment, glassware, and instruments.
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