2) You are trying to determine a TLC solvent system which will separate the compounds X, Y, and Z. You ran the compounds on a TL
C plate using hexanes/ethyl acetate 95:5 as the eluting solvent and obtained the chromatogram below. How could you change the solvent system to give better separation of these three compounds?
Thin layer chromatography is a chromatographic technique used to separate the components of a mixture using a thin stationary phase supported by an inert backing and a mobile phase. The separation principle is in the different affinities between the components of the mixture and the stationary or mobile phase.
<em>The affinity in mobile phase could be improved changing the polarity of this phase</em>. In this case, you could change proportion of hexane/ethyl acetate to change polarity of mobile phase and the affinity of the different compounds to mobile or stationary phase.
