Cloning is a process of producing genetically identical organisms through the techniques of biotechnology. Reproductive cloning of animals involves the somatic cell nuclear transfer in which the nucleus from an adult donor cell is transferred to an egg cell from which the nucleus has been removed. It is then incorporated into the uterus of a surrogate animal.
Pet cloning is not acceptable because of the following reasons-
(1) Its a very expensive procces with a high rate of failure.
(2) The clones produced tend to develop health problems. Large Offspring Syndrome (LOS) with very large organs is observed showing problems with blood flow and breathing with respect to heart and lungs, renal failure and other complicated issues.
(3) The clones are susceptible to skeletal abnormalities, higher rates of infection and tumour development. This results in a high mortality (death) rates.
(4) Since a surrogate mother is involved in the process of cloning, they experience a lot of health problems while carrying the embryos and sometimes it can be even killed.
Thus, cloning of pets is not acceptable as it affects both the cloned animal and the surrogate mother which is not preferred. Instead, adopting a homeless pet will provide shelter to another animal with a better nourishment.
DNA ligase is the enzyme that repair irregularities and breaks in the back bone of double stranded DNA. The enzyme function by sealing the repair in the DNA, it seals recombination fragments and it connects okazaki fragments during replication of DNA.
If a mutation reduce the amount of ligase, all the functions of the ligase enzyme listed above will be affected negatively.
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