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lesya692 [45]
3 years ago
15

Using site-directed mutagenesis, you have created a mutant form of chymotrypsin that has alanine substituted for the usual serin

e at position 195. Which of the following effects would your expect to observe? a. No effect or slight increase in affinity for substrate coupled to a complete loss of enzyme activity. b. A decrease in the affinity for substrate coupled to a decrease in enzyme activity. c. An increase in the rate of peptide-bond cleavage due to an increase in the rate of acid-base catalysis. d. An increase in the rate of peptide-bond cleavage due to an increase in the rate of covalent catalysis. e. A complete loss of enzyme activity due to the inability to bind substrate
Biology
1 answer:
tankabanditka [31]3 years ago
4 0

Answer:

The correct answer is - option E.

Explanation:

Serine may not be affected by the mutation as it has no part of the hydrophobic binding site so it won't affect the affinity of the binding of the substrate. The peptide bond-breaking would not occur without the catalytic end or part so the enzyme would be the complete loss of enzyme activity due to site-directed mutagenesis that is created from the chymotrypsin.

Thus, the correct answer is - option E.

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The answer is; A & C

The lagging strand is replicated in fragments called Okazaki fragments, each initiated by a primer. The fragments are later joined into one strand by DNA ligase. Replication occurs by adding nucleotides to the 3’ end of a preceding nucleotide. Because the lagging strand is antiparallel to the leading strand, the replication of the lagging strand is in the opposite direction as the replication fork direction. This is why the lagging strand is replicated in fragments because replication is being carried out by a single DNA polymerase (moving in the direction of the replication fork) per replication fork.  


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Answer:

<h2>Mitosis and meiosis are the ways of cell division which result daughter cells for growth, development and reproduction. </h2>

Explanation:

Mitosis and meiosis result daughter cells for growth, development and reproduction in the living world.

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Children aged 5 to 15 are most susceptible to developing scarlet fever. Scarlet fever has become less dangerous because to antibiotic treatments, despite once being thought of as a devastating pediatric ailment. Scarlet fever can nevertheless lead to more severe problems that impact the heart, kidneys, and other body organs if left untreated.

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