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lesya692 [45]
3 years ago
15

Using site-directed mutagenesis, you have created a mutant form of chymotrypsin that has alanine substituted for the usual serin

e at position 195. Which of the following effects would your expect to observe? a. No effect or slight increase in affinity for substrate coupled to a complete loss of enzyme activity. b. A decrease in the affinity for substrate coupled to a decrease in enzyme activity. c. An increase in the rate of peptide-bond cleavage due to an increase in the rate of acid-base catalysis. d. An increase in the rate of peptide-bond cleavage due to an increase in the rate of covalent catalysis. e. A complete loss of enzyme activity due to the inability to bind substrate
Biology
1 answer:
tankabanditka [31]3 years ago
4 0

Answer:

The correct answer is - option E.

Explanation:

Serine may not be affected by the mutation as it has no part of the hydrophobic binding site so it won't affect the affinity of the binding of the substrate. The peptide bond-breaking would not occur without the catalytic end or part so the enzyme would be the complete loss of enzyme activity due to site-directed mutagenesis that is created from the chymotrypsin.

Thus, the correct answer is - option E.

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Complete each sentence by putting the labels to the appropriate blanks. Then place each sentence in a logical order beginning wi
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Answer:

c. Beginning with the return from the systemic circulation, blood enters the <em>right atrium. </em>

a. Blood enters the right ventricle from the right atrium through the right atrioventricular valve which is also known as the <em>tricuspid valve</em>

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e. After circulating through the <em>pulmonary capillaries</em>, the blood returns to the <em>left atrium. </em>

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Explanation:

c. Beginning with the return from the systemic circulation, blood enters the <em>right atrium. </em>

a. Blood enters the right ventricle from the right atrium through the right atrioventricular valve which is also known as the <em>tricuspid valve</em>

d. The right ventricle contracts next, which forces blood up through the right semilunar valve which is otherwise known as the <em>pulmonary valve .</em>

e. After circulating through the <em>pulmonary capillaries</em>, the blood returns to the <em>left atrium. </em>

f. From the left atrium, blood moves through the left <em>atrioventricular valve </em>which is also referred to as the <em>bicuspid</em> or mitral valve.

b. From the left ventricle, the blood is pumped up to <em>aorta</em> the through the left semilunar valve which is sometimes referred to as the <em>aortic valve</em>.

3 0
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Which chemicals are used to cut DNA into fragments for a gel electrophoresis procedure
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Answer:

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Explanation:

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4 0
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