The molar mass of monotonic Nitrogen is 14 g/mol. Since this is diatomic Nitrogen, double that to 28 g/mol.
Next, divide total mass by molar mass, 500 g / 28 g/mol, which gives <span>17.8571 moles. A mole is defined as being 6.022*10^23 molecules, so multiply moles by molecules/mol (Avogadro's number), and we finally end up with something like 1.075 * 10^25, give or take a few billion particles.</span>
Answer is: Louis Pasteur (1822-1895) disproved spontaneous generation. He boiled liquid with proteins and other nutrients in S-neck flasks and regular flask to kill all microorganism present. In regular flask liquid showed changes when exposed to air and in S-neck flack there was no change (microorganism can not getting in the flask because the S- neck). When he removed S-neck, liquid changed because microorganisms came in liquid.
Pasteur showed it was not the air itself but particles within the air that had microorganisms.
