Answer:
C.The added epitope disrupts the function of the tagged protein
Explanation:
When an additional sequence is tagged to a protein to use comercial antibodies, there are several reasons why this procedure wouldn't work as expected (note that we're assuming the protein is being expressed but it's not possible to detect it).
For example, the sequence of nucleotide added to codify for the tagged epitope are removed during the RNA processing. In that case, the protein would be expressed without the epitope, so it would be impossible to localize it with the antibodies.
Also, it could be that the new epitope is affecting some way the protein folding, making it not functional. This way, it would be degraded by the cell so it wouldn't be detected.
Another possibility is that the epitope doesn't affects the protein folding nor its function, but during the folding ends up in a conformation that makes it inaccesible for the antibody.
In summary, the way as the possible answers to this question are shown, the correct option seems to be C:<em> The added epitope disrupts the function of the target protein.</em>
Answer:
1st C or D 2nd 3
Explanation:
Good Luck I hope this helped.
Answer:
All euglena have chloroplasts and can make their own food by photosynthesis. ... Euglena move by a flagellum (plural ‚ flagella), which is a long whip-like structure that acts like a little motor. The flagellum is located on the anterior (front) end, and twirls in such a way as to pull the cell through the water.
Explanation:
In general, flagellum is a long whip like organ which protrudes to the exterior from the cell body and permits mechanical work without any marked change in the form of the effector cell. In Euglena, there are two flagella. One of them is equal in length to body while other is short.
The effects of electrical stimulation on the sciatic nerve may be observed by watching the nerve under a dissecting microscope. Electric stimulation therapy is a therapeutic treatment that applies electrical stimulation in treating muscles spasm and pain. An assortment of lower back problems can cause the pain that radiates along the sciatic nerve.
Answer:
b.Movement of phospholipids in the plasma membrane from one monolayer to the other requires the polar or charged head groupd to enter the hydrophobic interior of the lipid bilayer. This process requires a large positive change in free-energy, thus making this type of movement unlikely without an input of energy
The plasma membrane serves a protective function for the cell and is a selective membrane for particles entry
