<u>α-1 Antitrypsin:</u> In biology, the term "α-1 Antitrypsin" is also referred as "A1AT" and is determined as a serpin that consists of a major "protective effects" against the cigarette smoking and involves the development of emphysema, along with that the patients with the mutations of the "A1AT gene" tends to display an increased risk of emphysema development. However, It is mostly developed in people who are younger in age ranging from 30 to 40 years.
The independent variable determines the value that needs to be calculated or the decision that needs to be made from the observations of the experiment. Hence, experimental design decision would most likely produce invalid and unreliable results when having more than one independent variable.