Answer and Explanation:
Bovine pancreatic trypsin inhibitor (BPT I) is a small globular protein and it inhibits the proteolytic enzymes like trypsin. BPTI is composed of ∝ helices, β sheets and 3 disulfide bonds. Due to these BPTI is a stable protein in its tertiary structure. It is almost inert to denaturation by urea and exhibits denaturation below 100 degree, only in highly acidic solutions. When all the disulfide bonds in BPTI are reduced, the protein is unfolded at room temperature and can reform three correct S-S pairings in native confirmation. if the 6 cysteine residues are reduced and unfolded in urea, the re-oxidation would yield 3 pairs with probability of first pair with 5, second pair with 3 and the third pair with 1 cysteine residues. Therefore, 5 x 3 x 1 = 15 combinations are possible accounting for 7% of protein refolding.
A complete collection of single-use and high-throughput electrocompetent and chemically competent E. coli
Choosing the ideal competent cells for your cloning applications and workflows is a critical component of success. Aspects to consider when selecting your competent E. coli strain include:
Transfection method – chemical versus electroporation
Transformation efficiency – bacterial strain and genotype
Downstream application – protein expression, library production, general cloning
The organism can be classified under Domain Eukarya or Eukaryote since it is made up of cells that have a nucleus enclosed within membranes. To be more specific, it can be placed under Kingdom Plantae which consists all organisms that are multicellular, eukaryotic, and photosynthetic.
Answer:
The correct answer is longer than those emitted by the sun.
Explanation:
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Answer:
1. Tundra Biome, 2. Rainforest Biome, 3. Grassland Biome, 4. Taiga Biome, 5. Desert Biome, 6. Deciduous Forest Biome.
Explanation:
1. Tundra is known for arctic conditions, lying north of Taiga biome. There are large ranges in most climatic conditions here.
2. Found around the equator, variety of tropical animals and plants.
3. Vegetation is mostly grass, very flat land thus good for farming.
4. Also known as the Boreal forest biome. Much vegetation.
5. High evaporation rate, animals adapted to dry conditions.
6. Cold winters and warm summers, thus winter makes growing deciduous.
