the correct answer is A- Reproductive isolation occurred through behavioral isolation
A process of formation of a new species from an existing species is called speciation. New species may formed due to geographical or reproductive barriers which isolates the members of a population of a species to form a new species.
In the given question, speciation in In the leopard frogs occurred due to a particular or specific call given by males to the females. The courtship call lead to reproductive isolation due to change in behavior. Such type of behavioral change will help in recognizing mates of the same species and this will act as a reproductive barrier among the other related species.
The relationship between the lithosphere and the asthenosphere is that they both make up the earth's mantle. The asthenosphere lies right below the lithosphere.
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I THINK it would be a thunderstorm because they usually do go out during that time