Answer:
A breakdown of the breaking buffer was first listed with its respective component and their corresponding value; then a table was made for the stock concentrations in which the volume that is being added was determined by using the formula 
. It was the addition of these volumes altogether that make up the 0.25 L (i.e 250 mL) with water
Explanation:
Given data includes:
Tris= 10mM
pH = 8.0
NaCl = 150 mM
Imidazole = 300 mM
In order to make 0.25 L solution buffer ; i.e (250 mL); we have the following component.
Stock Concentration Volume to be Final Concentration
added
1 M Tris 2.5 mL 10 mM
5 M NaCl 7.5 mL 150 mM
1 M Imidazole 75 mL 300 mM
. is the formula that is used to determine the corresponding volume that is added for each stock concentration
The stock concentration of Tris ( 1 M ) is as follows:
.
The stock concentration of NaCl (5 M ) is as follows:
.
The stock concentration of Imidazole (1 M ) is as follows:
.
Hence, it is the addition of all the volumes altogether that make up 0.25L (i.e 250 mL) with water.
Using Gay-Lussac's Law, pressure is proportional to (absolute) temperature in Kelvin. We first convert the temperature values to Kelvin: 110 C = 383.15 K, while 65 C = 338.15 K.
P1/T1 = P2/T2
22.5/383.15 = P2/338.15
P2 = 19.9 psi
The most accurate measurement is 1.1 g. Option A
<h3>What is accuracy?</h3>
The term accuracy refers to the fact that the measurement is close to the true value. The closer the measurement is to the true value as given, the more accurate it is.
In this case, the true value of the mass of the sample of calcium carbonate is 1.134 g. Now we have to look at all the masses of as obtained by Emma during the experiment.
The most accurate measurement is 1.1 g. Option A
Learn more about accuracy:brainly.com/question/15276983
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