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3 years ago

How many colors would you see if you were analyzing a pure substance through chromatography?

Chemistry

2 answers:

harina [27]3 years ago

4 0

Answer :

The correct answer is no color will observed if a pure substance is analyzed ,

Chromatography :

It is a technique commonly used to separate a mixture of chemical substances based on their affinity with stationary phase and mobile phase .

The mobile phase travels through the stationary phase in a definite direction . The component of mixture travels along with mobile according to their affinity , ionic interactions , diffusion , solubility on stationary phase .

The component has more affinity with mobile phase goes higher with mobile phase .

While component with stationary phase remain at lower position on stationary phase .

This is how separation of components takes place.

A pure substance has only one type of component in it . There is no mixture of components.

Hence when pure substance will be analyzed by chromatography technique, there will be no separation of components .

So there will no color be observed.

AfilCa [17]3 years ago

3 0

For separation of components of mixture chromatography technique is used. Parts of mixture are separated by using the different solubilities of the parts for a stationary phase (that does not move) compared to the mobile phase (that carries the parts). Due to capillary action, the solvent will move up with paper or plate in apparatus. The mobile phase will move with solvent and the stationary phase will remain adhere to the paper or plate. And thus, giving separate colors for the substances present in the mixture.

Hence, for pure substance, only one color would be observed through chromatography.

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fiasKO [112]

Answer:

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3 0

3 years ago

What volume of 0.307 m naoh must be added to 200.0ml of 0.425m acetic acid (ka = 1.75 x 10-5 ) to produce a buffer of ph = 4.250

Blababa [14]

The buffer solution target has a pH value smaller than that of pKw (i.e., pH < 7.) The solution is therefore acidic. It contains significantly more protons $\text{H}^{+}$ than hydroxide ions $\text{OH}^{-}$ . The equilibrium equation shall thus contain protons rather than a combination of water and hydroxide ions as the reacting species.

Assuming that $x \; \text{L}$ of the 0.307 $\text{mol} \cdot \text{dm}^{-3}$ sodium hydroxide solution was added to the acetic acid. Based on previous reasoning, $x$ is sufficiently small that acetic acid was in excess, and no hydroxide ion has yet been produced in the solution. The solution would thus contain $0.2000 \times 0.425 - 0.307 \; x = 0.085 - 0.307 \; x$ moles of acetic acid and $0.307 \; x$ moles of acetate ions.

Let $\text{HAc}$ denotes an acetic acid molecule and $\text{Ac}^{-}$ denotes an acetate ion. The RICE table below resembles the hydrolysis equilibrium going on within the buffer solution.

$\begin{array}{lccccc}\text{R} & \text{HAc} & \leftrightharpoons & \text{H}^{+} & + & \text{Ac}^{-}\\\text{I} & 0.085 - 0.307 \; x& & 0 & & 0.307 \; x\\\end{array}$

The buffer shall have a pH of 4.250, meaning that it shall have an equilibrium proton concentration of $10^{4.250}\; \text{mol}\cdot \text{dm}^{-3}$ . There were no proton in the buffer solution before the hydrolysis of acetic acid. Therefore the table shall have an increase of $10^{-4.250}\;\text{mol}\cdot \text{dm}^{-3}$ in proton concentration in the third row. Atoms conserve. Thus the concentration increase of protons by $10^{-4.250}\;\text{mol}\cdot \text{dm}^{-3}$ would correspond to a decrease in acetic acid concentration and an increase in acetate ion concentration by the same amount. That is:

$\begin{array}{lcccccc}\text{R} & \text{HAc} & \leftrightharpoons & \text{H}^{+} & + & \text{Ac}^{-}\\\text{I} & 0.085 - 0.307 \; x& & 0 & & 0.307 \; x\\\text{C} & - 10^{-4.250} & & +10^{-4.250} & & +10^{-4.250} \\\text{E} & 0.085 - 10^{-4.250} - 0.307 \; x& & 10^{-4.250} & & 10^{-4.250} + 0.307 \; x\end{array}$

By definition:

$\text{K}_{a} = [\text{H}^{+}] \cdot [\text{Ac}^{-}] / [\text{HAc}]\\\phantom{\text{K}_{a}} = 10^{-4.250} \times (10^{-4.250} + 0.307 \; x) / (0.085 - 10^{-4.250} - 0.307 \; x)$

The question states that

$\text{K}_{a} = 1.75 \times 10^{-5}$

such that

$10^{-4.250} \times (10^{-4.250} + 0.307 \; x) / (0.085 - 10^{-4.250} - 0.307 \; x) = 1.75 \times 10^{-5}\\6.16 \times 10^{-5} \; x = 1.48 \times 10^{-6}\\x = 0.0241$