Question

Describe how you would use the yeast-two-hybrid technique to find the proteins that interact with a liver-specific receptor protein.

Answer 1

Answer:

Yeast-two-hybrid technique is one of the most important molecular technique that can be used to study the interactions at the molecular level. The interactions between the protein and DNA-protein  interaction can be studied by this technique.

If the individual wants to study the liver liver-specific receptor protein then yeast-two-hybrid technique can be applied. The interested protein that can acts as prey and bait proteins must be covalently linked with the other third protein known as the reporter protein.  Then the activity of the reporter protein is studied with the interactions of the prey and bait protein.