Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Answer:
Frequency of allele A1- 0.41
Explanation:
In Hardy weinberg equilibrium,
P refers to the dominant allele
q refers to the recessive allele
The allele frequency will be p+q=1
The genotypic frequency is- P²+q²+2pq=1
P²= genotype of dominant trait ( A1A1)- 77
2pq= genotype of heterozygotes (2pq)- 65
q²= genotype of recessive trait (A2A2)- 123
Total number of offsprings= 77+ 65+ 123
= 265
Now to calculate allele frequency of A1=
= 77/265 + 1/2( 65//265)
= 0.290+ 0.122
= 0.413
Thus, 0.41 is correct.
Answer:
Exercising
Explanation:
This happens because, when you exercise, the blood vessels in your muscles dilate (vasodilation) and as a consequence, the rate of blood flow is faster. The increased blood flow delivers more oxygenated blood to the working muscle.
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Hoped it helped!
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