Some codons are sup pressed, so they do not appear to be producing any proteins. These amino acids may be part of such codons.
Answer:
tetracycline
Explanation:
When the DNA insert is ligated in Pstl site, the ampicillin gene is disrupted while <em>tetracycline gene is still intact</em>.
- If the bacterial colony is growth in a medium with only ampicillin, only the cells that contains the plasmid with the insert will grow.
- If the bacterial colony is growth in a medium with only tetracycline, the cells which have incorporated the plasmid will survive. We cannot distinguish between cells with plasmid + insert and plasmid alone.
- If the bacterial colony is growth in a plate with ampicillin + tetracyclin, only the cells with the plasmid without the insert will grow.
Therefore, to ensure that the cells of a bacterial colony contains the plasmid, they have to be seeded in a plate with tetracycline.
