The Kirby–Bauer test or the disk diffusion test, is a method to determine the antibiotic sensitivity of the given bacteria. This test involves the use of antibiotic discs to determine the effect of antibiotics on the bacteria.
In this test, the wafers having antibiotics and the bacteria are placed on the agar plate and incubated. If the antibiotics present stops the growth of the bacteria, there will be an area around wafer with no bacterial growth, such an area is known as the zone of inhibition.
<u>The </u><u>diameter of this zone of inhibition</u><u> is measured to determine the </u><u>antibiotic sensitivity of the given bacteria</u><u>.</u>
The oxidation of organic compounds by molecular oxygen to produce CO2 and water releases free energy because electrons are being moved from atoms that have a lower affinity for electrons.
