When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
Answer: C. On a sloped parking lot
Surface runoff can be referred as the flow of water, that occurs when the excess water flows from the storm water, melt water, flood, rain streams and rain fall. The fast moving water current takes along with it superficial layers like surface and sub- surface layers of soil. Hence, causes water erosion of soil.
The slope parking lot will favor, surface runoff more likely than in a grassy prairie, mulched flower bed, or in a sand pit because the slope will provide a surface for free flow of water. There are chances that water gets absorbed by the soil in the grassy prairie, mulched flower bed and sand pit. Whereas the slopy surface of parking lot will not allow the water retention by soil, instead will accelerate the flow of water.
Answer:
Soil erosion causes siltation in rivers, dams, etc. In some areas, huge river deltas are formed (such as River Nile delta) because of soil erosion. Such places are good for agriculture but not good for urbanization. However, some people do not care about it, they just want to construct buildings on alluvium type soils.
