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We (humans) remove nutrients from the land and discharge them into aquatic environments. On one hand, this leads to soil depletion on the land, and on the other hand, an over abundance of the nutrients and pollution of water sources.
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cast
Cast fossils are the type people are most familiar with, as they make up the spectacular dinosaur skeletons that on view in museums. Cast fossils occur when minerals deposit into the mold left by the rotting organic material, resulting in a three-dimensional replica of the hard structures of the plant or animal
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A quadrat is a frame (usually a square or a circle) of known area used to isolate a subset of the population. This subset will comprise one sample. Quadrats come in various sizes (and shapes) with the size selected determined by features of the organisms in the population to be sampled.
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One of the main reasons why there is a need to create additional three kingdoms was due to the invention of the microscope. The scientific device paved way for a more intensive study of the tiny organisms most considered as single-celled which cannot be seen by the naked eye.