Question

Describe the principal and procedure of spiral plating.

Answer 1

Answer and Explanation:

Principal of Spiral plating:In the spiral plate technique, a mechanical plater vaccinates the fluid sample on a pivoting plate agar. The volume of fluid sample decline from the inside to the edge of the plate. The microbial concentration is controlled by checking the provinces on a piece of the Petri dish.

A technique, portrayed for deciding the quantity of microorganisms in an solution by the utilization of a machine which stores a known volume of test on a pivoting agar plate in a consistently diminishing sum as an Archimedes spiral.

Materials used: agar plates, dilution blanks, pipettes

Method:

A spiral plater is a gadget that disperses a constantly diminishing volume of fluid over a solitary pivoting agar plate (the apportioning arm moves like a needle on a turntable, just in reverse). The agar is then hatched and checks are made. It can viably convey up to a 105 concentration range on one plate, yet list requires an uncommon tallying lattice. After the example is brooded, distinctive state densities are clear on the outside of the plate. A changed checking matrix is portrayed which relates zone of the plate of volume of test. By checking a proper territory of the plate, the quantity of microscopic organisms in the example is assessed.