Living things consists of the organisms like the plants, animals, fungi, protozoa and bacteria. These animals vary in the complexity of the body and have different number of cells in their body. Organisms like the plants and animals are made up of several different kinds of cells. These organisms are called multi-cellular organisms. On the other hand, organisms like the baker's yeast (a fungi) and a plethora of bacteria are single celled organisms. They are called unicellular organisms. Hence we can say that the living things are made up of at least one cell.
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The four limitations are
1.It's often impossible to repeat trials on the same subjects.
2-Subjects may report an inaccurate medical history.
3-It can be difficult to control all possible variables.
4-It's impossible to come up with testable scientific questions for human subjects.
Research involving human beings is ultimately necessary for improvements in human health and welfare. Any results about healthy physiology, illness causes, treatment effectiveness, learning, or behavior must be verified by carefully controlled investigations involving human participants. Sadly, not all human research have been valid and beneficial. It is possible to harm people in the name of research. The most well-known instances happened in Nazi Germany. Investigations conducted after the war found several crimes, including experiments in which participants were submerged in extremely cold water to determine how long it would take them to pass away from hypothermia.
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