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Answer:
The plate that contains Kanamycin in it will have growth of colonies of bacteria that have been transformed with the plasmid which has the kanamycin-resistant gene. The bacterial cells that did not get transformed with this plasmid will not be able to grow into colonies in the presence of kanamycin.
While the negative control, a plate with any other antibiotic,such as ampicillin or tetracyclin, added to it will not allow growth of any bacteria on it.
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Answer:
The CFTR behaves like a channel for chlorine. Its dysfunction affects both the transport of this ion and other ions and the transport of water, which causes a thickening of secretions, an alteration of mucociliary transport and local defenses, facilitating bacterial colonization and promoting the release of pro-inflammatory mediators in the airway
Explanation:
CFTR is a protein expressed in the epithelial cells of the respiratory system, pancreas, bile ducts, sweat glands and genitourinary system. It is made up of a single chain made up of 1,480 amino acids. It contains 12 hydrophobic regions embedded in the lipid membrane and acts as a channel for chlorine.The highest levels of expression of the CFTR protein are found in serous cells of the submucosal glands of the proximal airway. In them, Cl- is released to the outside. In addition, there are channels for Na +, through which this ion is also secreted in the same direction. These movements cause the displacement of water and also of mucins, originating in the submucosal glands, allowing their presence on the surface of the airway. For all this to occur normally, a basolateral Na + - K + - ATPase cotransporter must function, another basolateral cotransporter formed by Na +, K + and 2 Cl-, which allows the latter to enter the cell, and an apical CFTR channel through which it exits the Cl- of the cell towards the acinar lumen. Na + leaves the cell following Cl- by a paracellular pathway accompanied by water. When CFTR malfunctions, Cl- does not exit through this channel and this implies a decrease in Na + and water in the canalicular lumen, with the consequent thickening of secretions.
This answer is dandelion because i seen the answer to this in my book yesterday!
