Question

When measuring the concentration of the blue dye you get a reading of 3.0 for an initial stock solution, and again after it is diluted. Explain why this result could occur

Answer 1

Explanation:

The beer - lambert law measures the amount of radiation being absorbed when it is passed through the solution .

This formula is as follows -

A= log₁₀ ( I₀ / I ) = Elc

Where,

E = is abosorption co-efficient

c = Concentration of the solution,

l = Length of the light path

In case of the stock solution, the concentration of blue dye is very high and we see absorbance nearly at 3.0 . But when we dilute the solution, the moles of blue dye remains same as before ( i.e as in stock solution) .

Hence ,

The radiation passing through the solution will eventually interact with same number of moles of blue dye in diluted solution and give absorbance nearly 3.0