Question

A standard PCR cycle includes three steps: denaturation (95°C), annealing (55°C), and elongation (65°C).

Answer 1

Answer:

Denaturation process: The DNA template  

Annealing process: Primers  

Elongation process: dNTPs and Taq polymerase

Explanation:

For the denaturing process, the only ingredient that is required is the DNA template that will be separated from a double helix (or double strand) into a single strand, by increasing the temperature to 95 C, (at this temperature the hydrogen bonds that keep together the double stranded break). After the double strand is denatured, the following process is annealing. For this, the required ingredient are the primers; these primers will hybridize or anneal according to the nucleotide complementarity to the single strand of the DNA. Finally, for the Elongation process, you will require the Taq polymerase and the dNTPs. The enzyme will synthesize or “generate” a new strand of DNA based on the DNA template, using the provided dNTPs in the direction 5’ to 3’.

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