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erik [133]
2 years ago
10

Which procedure would help ensure that a foreign DNA fragment could easily incorporate into a bacterial plasmid when the two are

mixed together?
a)The fragment and plasmid are both cut with the same restriction enzyme.
b)The plasmid is modified to contain GFP.
c)The plasmid is pretreated with DNA ligase.
d)The DNA fragment is pretreated with DNA ligase.
Biology
1 answer:
rosijanka [135]2 years ago
5 0
The correct answer is a !!

Both should be cut with same restriction enzyme to have sticky ends so that they easily combine with each other !
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Explanation:

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People who claimed to have experienced the presence of the huge ape-like creature named Big Foot indicate that one specific qual
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Answer:

D. unnatural scream

Explanation:

The Bigfoot is a an animal around there's lot of debate, as there are thousands of sightings, but not conclusive proof that it actually exists, thus it falls into the category of mythological creatures for the time being. This creature has been described by thousands of people that claim to have seen it or hear it, and the descriptions are pretty much the same, very tall, hominid-like, bipedal, dark fur, very quick, and terrifying. Something else that is a specific feature of the creature is the sounds its make, as everyone has said that it produces incredibly powerful unnatural sounding scream. People have said that they have frozen from fear after hearing those sounds, and that whenever that sound occurs all other animals run away frightened in the opposite direction.

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Indicate whether each of the following statements is true of depurination (DP), deamination (DA), or pyrimidine dimer formation
solniwko [45]

Answer:

- This process is caused by spontaneous hydrolysis of a glycosidic bond: depurination and deamination

- This process is induced by ultraviolet light:  pyrimidine dimer formation

- This can happen to guanine but not to cytosine: depurination

- This can happen to thymine but not to adenine:  pyrimidine dimer formation

- This can happen to thymine but not to cytosine: none

- Repair involves a DNA glycosylase: deamination

- Repair involves an endonuclease: depurination, deamination and  pyrimidine dimer formation

- Repair involves DNA ligase: depurination, deamination and  pyrimidine dimer formation

-  Repair depends on the existence of separate copies of the genetic information in the two strands of the double helix: depurination, deamination and  pyrimidine dimer formation

- Repair depends on cleavage of both strands of the double helix: none

Explanation:

Depurination is the loss of purine bases (either adenine or guanine), while deamination refers to the removal of an amino group. During depurination, a β-N-glycosidic bond is cleaved by hydrolysis and a nucleic base is released (either adenine or guanine). All DNA bases may undergo deamination, except thymine (since thymine does not have an amino group). The ultraviolet (UV) radiation can cause thymine or cytosine to form dimers (e.g., pyrimidine dimers), being thymine dimers the most common lesion when DNA is exposed to UV light. Pyrimidine dimers may be repaired by different excision mechanisms, e.g., nucleotide excision repair, where the recognition of the DNA damage leads to the removal of the DNA fragment containing the lesion. DNA glycosylases are enzymes involved in the mechanism of base excision, these enzymes recognize and remove damaged bases by hydrolysis of the glycosidic bond, producing an abasic (apurinic and apyrimidinic) site. A DNA ligase enzyme covalently joins two DNA molecules by forming a phosphodiester bond, which is required during these processes.

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