Answer:
Explanation:
Bacterial count in stock- 1.85x10^6 cfu/ml
Dilution methods
Take 100 uL or (0.1ml) from stock and add to 900ul (0.9ml) saline and mixed it- this makes 10^1dilution.
Now take 100ul from 10^1 dilution and add to next 900ul saline this is 10^2 dilution, similarly do upto 10^5 dilution.
Then take 100ul from 10^ 4 and 10^5 dilution seperately and plate on LB agar plate seperetely and count the colonies.
Cfu/ml formula= (No.of colonies x dilution factor)/0.1 ml
So suppose, 18 colonies formed on 10^4 dilution then total no. Of cells in stock will be 18x10^4/ 0.1= 18x10^5 cfu/ml.
If we dilute 10^4 or 10^5 that's leads to colony count of 18-19 colonies on 10^4 dilution while 2 colonies should come on plate of 10^5 dilution.
Answer:
El almacenamiento a largo plazo puede ser similar al aprendizaje: el proceso mediante el cual la información que puede ser necesaria nuevamente se almacena para su recuperación bajo demanda. El proceso de localizar esta información y traerla de vuelta a la memoria de trabajo se denomina recuperación.
Explanation:
ojala te sirva es lo unico que se :D
There would not be enough nutrients in the ground so the grass would possibly die
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Answer:
Mississippian fossils are abundant in portions of the Midwest and South and include vast beds of limestone and marble.
Explanation:
For example, the domed ceiling of the Jefferson Memorial in Washington, D.C., is made of Indiana limestone that was deposited during the Mississippian Period.By the end of the Carboniferous, reptiles had migrated well toward the interior of Pangea. These early pioneers went on to spawn the archosaurs, pelycosaurs, and therapsids of the ensuing Permian period. (It was the archosaurs that went on to spawn the first dinosaurs nearly a hundred million years later.)
