When the Total cholesterol level is 240 mg/dL and above
Explanation:
Lipids in our body is classified as LDL-cholesterol, HDL- cholesterol and triglyceride. The human body lipidi content is measured as lipid profile that is the total cholesterol level, LDL-cholesterol, HDL- cholesterol and triglyceride.The lipid value in our body is said to be non desirable when the Total cholesterol level is 240 mg/dL and above, LDL cholesterol is 190 mg/dL and above, HDL cholesterol is 60 mg/dL and above, and when the triglycerides level is 500 mg/dL and above
The organism previously used 15N for replication so all the DNA molecules were of 15N15N type. Then the organism is shifted to a medium where only 14N is available for replication.
According to semi conservative mode of replication, a newly synthesised DNA molecule consists of one new strand and one parental strand. So after the first round of replication, All the 15N strands will synthesise new DNA strands using 14N resulting into intermediate 15N14N DNA molecules. Hence, only one band would be observed (15N14N) above the original 15N15N band since 15N14N has lighter isotope too so it will be lighter than 15N15N molecules and will lie above it.
After second round of replication, 15N strand from 15N14N would synthesise another 14N strand. 14N strand from 15N14N molecules will also synthesise another 14N strand. So now, 50% of the DNA molecules will be of 15N14N intermediate type and 50% of them will be of 14N14N type.Two bands will be observed above the original 15N15N band. One band of 15N14N molecules will be right above it and other band of 14N14N molecules will be even higher because it is the lightest band since it has only the lighter isotope of nitrogen.
