If one wishes to clone a gene using typical restriction endonucleases, how does the restriction endonuclease identify the appropriate cut sites in the genome using the palindromic sequence.
Restriction endonucleases are a family of enzymes usually found in bacteria that cuts DNA at a specific sequence called recognition site. There are three type of restriction endonuclease Type I, Type II, and Type III.
Each endonuclease recognizes a particular sequence called palindromic sequence which is a sequence that reads the same sequence in both the strands either reading from the 3 prime end or 5 prime end.
The endonuclease cuts the palindromic sequence in such a way that it produces sticky ends.
To learn more about restriction endonucleases here
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There isn't exactly an opposite of weathering, although I would say that it is sedimentation/ deposition, since it builds up rock and dirt and such, rather than breaking it down.
Answer:
the nucleus regulates cell activity :)
Temperature is the one option among the given options that does not contribute to soil consistence. The correct option among all the options that are given in the question is the second option or option "b". Soil consistency is basically the resistance of the soil against mechanical stress, manipulations and various moisture contents.