Answer:
Glucose metabolism is impeded as the conversion of fructose-6-phosphate to fructose-2,6-bisphosphate in glycolysis is impossible after a knockout in mice.
On glucose levels, glucose levels rise as the feedback mechanism set in place to control the metabolism of glucose to ATP is absent.
Explanation:
GLUCOSE METABOLISM
PFK-2/FBPase-2 is an enzyme involved in regulating glycolysis and gluconeogenesis. It is found abundantly in organs such as the liver and heart.
It is a rate limiting enzyme critical in the catabolism of glucose which produces energy in the body.
Phosphofructokinase-2 (PFK2) catalyzes the conversion of fructose-6-phosphate to fructose-2,6-bisphosphate. PFK-2 is regulated by glucagon in the liver, epinephrine in muscle and by insulin, glucagon and epinephrine being hormones.
A knockout in mice, will conversely lead to an increased level of fructose-6-phosphate in glycolysis and with a lack of the rate limiting enzyme, glucose levels rise, leading to a consequential rise in glucose levels.
The rate limiting enzyme functions in the body's natural feedback mechanism to regulate the production of energy as well as the release of glucose for the production of energy.
