Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
The Sun's outer gases extend far beyond the photosphere (Figure 6). Because they are transparent to most visible radiation and emit only a small amount of light, these outer layers are difficult to observe. The region of the Sun's atmosphere that lies immediately above the photosphere is called the chromosphere.
<h2>Problem of protein alters the movement of Ions </h2>
Explanation:
- Proteins are tiny machines that do explicit occupations inside a cell. The directions for building every protein are encoded in DNA. Proteins are gathered from building squares called amino acids.
- The CFTR protein is comprised of 1,480 amino acids. When the CFTR protein chain is made, it is collapsed into a particular 3-D shape. The CFTR protein is formed like a cylinder that experiences the film encompassing the cell, similar to a straw experience the plastic top on a cup.
- In individuals with CF, changes in the CFTR quality can cause the accompanying issues with the CFTR protein:
- It doesn't function admirably
- It isn't delivered in adequate amounts
- It isn't delivered in any way
- At the point when any of these issues happen, the chloride particles are caught inside the cell, and water is never again pulled in to the space outside the cell. When there is less water outside the cells, the bodily fluid in the aviation routes gets got dried out and thickens, making it smooth the cilia. The cilia can't clear appropriately when thick, clingy bodily fluid overloads them.
Answer: The answer is C!!! :D maintaining homeostasis by regulating the body's temp!
Explanation:
Shivering as a natural, involuntary response to generate heat is an example of maintaining homeostasis by regulating body temperature.
Homeostasis refers to the maintenance of a relatively constant body environment. The normal range of operation of the body system is known as the setpoint.
When the setpoint temperature for some animals is breached, a negative feedback mechanism is used to return it to the setpoint. Shivering to generate heat is a response to a cold environment when the body's temperature is about to drop below the setpoint.
The oppositeis sweating. Sweating causes cooling and comes in response to when the setpoint temperature is exceeded.
Hopes this helps happy early Christmas!!!! :D
